KMID : 0043320110340020223
|
|
Archives of Pharmacal Research 2011 Volume.34 No. 2 p.223 ~ p.228
|
|
Anti-allergic activity of sesquiterpenes from the rhizomes of Cyperus rotundus
|
|
Jin Jeong-Ho
Lee Dong-Ung Kim Yeong-Shik Kim Hyun-Pyo
|
|
Abstract
|
|
|
From the 70% ethanol extract of the rhizomes of Cyperus rotundus (CRE), several major constituents including the sesquiterpene derivatives (valencene, nootkatone, and caryophyllene ¥á-oxide), monoterpenes (¥â-pinene, 1,8-cineole, and limonene) and 4-cymene were isolated and examined for their anti-allergic activity in vitro and in vivo. In rat basophilic leukemia (RBL)-1 cells, the sesquiterpenes strongly inhibited 5-lipoxygenase-catalyzed leukotrienes production. In addition, they inhibited ¥â-hexosaminidase release by antigen-stimulated RBL-2H3 cells, with valencene having the highest inhibitory effect. CRE inhibited leukotrienes production and ¥â-hexosaminidase release at 300 ¥ìg/mL. It was also found that the most active sesquiterpene (valencene) and CRE inhibited ¥â-hexosaminidase degranulation by inhibiting the initial activation reaction, Lyn phosphorylation, in IgE-stimulated RBL-2H3 cells. Moreover, CRE, valencene and nootkatone significantly inhibited the delayed-type hypersensitivity reaction in mice when administered orally at 50?300 mg/kg. In conclusion, C. rotundus and its constituents, valencene, nootkatone, and caryophyllene ¥á-oxide, exert anti-allergic activity in vitro and in vivo. These sesquiterpenes, but not monoterpenes, certainly contribute to the anti-allergic activity of the rhizomes of C. rotundus.
|
|
KEYWORD
|
|
Cyperus rotundus, Valencene, 5-Lipoxygenase, ¥â-Hexosaminidase, Anti-allergy
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|